Coding

Part:BBa_K5048051:Design

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


ccdB-dt-Plux-ccdA-dt-J23106-LuxR-dt-J23106-lasl-dt


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2082
    Illegal PstI site found at 2270
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2082
    Illegal NheI site found at 897
    Illegal NheI site found at 920
    Illegal NheI site found at 1839
    Illegal NheI site found at 1862
    Illegal PstI site found at 2270
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2082
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2082
    Illegal PstI site found at 2270
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2082
    Illegal PstI site found at 2270
    Illegal NgoMIV site found at 2336
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


Design Notes

We design this part in order to reproduce the result of the literature, except for the double terminators. we discussed with CDG China and they recommended this part because it had high efficiency.



Source

complete synthesis

References